Description
Luciferinsarelight-emittingheterocycliccompoundscommonlyusedinbiochemistry.Luciferinisthenaturalligandfortheenzymeluciferase,whichoxidizesthesubstrate,emittingenergyintheformoflightintheprocess.FireflyluciferaserequiresATPforitsreaction,emittingagreenish-yellowluminescenceatapeakwavelengthofapproximately530nm.ThepotassiumandsodiumsaltsofluciferindissolvemuchmorereADIlyinwaterortypicalbuffersandmaybemoreconvenientthanthefreeacidformforinvivoimaging.