Description
PuromycinisanaminonucleosideantibioticcompoundoriginallyproducedbyStreptomycesalboniger.Puromycindisplaysantibacterialactivitythroughinhibitionofribosomalproteintranslation;itresemblesthe3’endoftRNAandisincorporatedintogrowingproteinchainsthroughtheribosomalAsite,inducingprematurechaintermination.PuromycinalsoinducesDNAdamagemediatedbyROSandoxidativestressinanimalmodels.Invitro,puromycininhibitsinsulin-stimulatedglycolysisbyinhibitinginsulinactivationofphosphofructokinase2.PuromycinalsoinhibitsdipeptidylpeptidaseII(DPP2;serinepeptidase)andmetallopeptidase.Additionally,thiscompoundinducesERKactivation-dependentapoptosisandmTOR-dependentautophagyinpodocytes,leADIngtoproteinuriaandglomerulardamage.
References
KangYL,SaleemMA,ChanKW,etal.Thecytoprotectiveroleofautophagyinpuromycinaminonucleosidetreatedhumanpodocytes.BiochemBiophysResCommun.2014Jan10;443(2):628-34.PMID:24333414.
LiuS,DingJ,FanQ,etal.Theactivationofextracellularsignal-regulatedkinaseisresponsIBLeforpodocyteinjury.MolBiolRep.2010Jun;37(5):2477-84.PMID:19728154.
MarshallCB,PippinJW,KrofftRD,etal.Puromycinaminonucleosideinducesoxidant-dependentDNAdamageinpodocytesinvitroandinvivo.KidneyInt.2006Dec;70(11):1962-73.PMID:17035936.
ProbstI,QuentmeierA,SchweickhardtC,etal.StimulationbyinsulinofglycolysisinculturedhepatocytesisattenuatedbyextracellularATPandpuromycinthroughpurine-dependentinhibitionofphosphofructokinase2activation.EurJBiochem.1989Jun15;182(2):387-93.PMID:2525468.
